| Registro completo |
| Provedor de dados: |
ArchiMer
|
| País: |
France
|
| Título: |
Exposure to the Neurotoxic Dinoflagellate, Alexandrium catenella, Induces Apoptosis of the Hemocytes of the Oyster, Crassostrea gigas
|
| Autores: |
Medhioub, Walid
Ramondenc, Simon
Vanhove, Audrey
Vergnes, Agnes
Masseret, Estelle
Savar, Veronique
Amzil, Zouher
Laabir, Mohamed
Rolland, Jean-luc
|
| Data: |
2013-12
|
| Ano: |
2013
|
| Palavras-chave: |
Shellfish
Toxins
Apoptosis
Gene expression
|
| Resumo: |
This study assessed the apoptotic process occurring in the hemocytes of the Pacific oyster, Crassostrea gigas, exposed to Alexandrium catenella, a paralytic shellfish toxins (PSTs) producer. Oysters were experimentally exposed during 48 h to the toxic algae. PSTs accumulation, the expression of 12 key apoptotic-related genes, as well as the variation of the number of hemocytes in apoptosis was measured at time intervals during the experiment. Results show a significant increase of the number of hemocytes in apoptosis after 29 h of exposure. Two pro-apoptotic genes (Bax and Bax-like) implicated in the mitochondrial pathway were significantly upregulated at 21 h followed by the overexpression of two caspase executor genes (caspase-3 and caspase-7) at 29 h, suggesting that the intrinsic pathway was activated. No modulation of the expression of genes implicated in the cell signaling Fas-Associated protein with Death Domain (FADD) and initiation-phase (caspase-2) was observed, suggesting that only the extrinsic pathway was not activated. Moreover, the clear time-dependent upregulation of five (Bcl2, BI-1, IAP1, IAP7B and Hsp70) inhibitors of apoptosis-related genes associated with the return to the initial number of hemocytes in apoptosis at 48 h of exposure suggests the involvement of strong regulatory mechanisms of apoptosis occurring in the hemocytes of the Pacific oyster.
|
| Tipo: |
Text
|
| Idioma: |
Inglês
|
| Identificador: |
http://archimer.ifremer.fr/doc/00176/28744/27213.pdf
DOI:10.3390/md11124799
|
| Editor: |
Mdpi Ag
|
| Relação: |
http://archimer.ifremer.fr/doc/00176/28744/
|
| Formato: |
application/pdf
|
| Fonte: |
Marine Drugs (1660-3397) (Mdpi Ag), 2013-12 , Vol. 11 , N. 12 , P. 4799-4814
|
| Direitos: |
2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
|
|
